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Acta Agronómica

Print version ISSN 0120-2812

Acta Agron. vol.71 no.4 Palmira Oct./Dec. 2022  Epub Apr 08, 2024

https://doi.org/10.15446/acag.v71n4.105631 

Articles

Fatty acid composition in Ocotea caudata (Nees) Mez (Lauraceae) fruits from Colombia

Composición de ácidos grasos en frutos de Ocotea caudata (Nees) Mez (Lauraceae) procedentes de Colombia

Elizabeth Gil-Archila1 

Wilman Antonio Delgado-Ávila2 

Luis Gonzalo Sequeda-Castañeda1 

Luis Enrique Cuca-Suárez2 

1Pontificia Universidad Javeriana. Bogotá, Colombia. egil@javeriana.edu.co, lsequeda@javeriana.edu.co

2Universidad Nacional de Colombia - Sede Bogotá. Bogotá, Colombia. wadelgadoa@unal.edu.co; lecucass@unal.edu.co


Abstract

The genus Ocotea of the Lauraceae family has species whose wood is employed in construction and for other purposes including as biofuel, for disinfection, cosmetics, and in the treatment of various diseases. Studies regarding Ocotea caudata’s phytochemical and pharmacognostic uses are scarce, thus, this work is the first to study its fruits. O. caudata (Nees) Mez fruits from Colombia were investigated for their oil content. Heptane extraction from O. caudata fruit oil yielded 54.9 % of total lipids, where the fatty acid composition was determined. The oil analysis by gas chromatography with flame ionization detection (GC-FID) demonstrated that lauric acid was the most abundant fatty acid (51.7 %, C12:0). In addition to lauric acid, oleic acid (23.6 %, C18:1) and palmitic acid (16.6 %, C16:0) were found. The fatty acid composition was confirmed by gas chromatography-mass spectrometry (GC-MS). O. caudata can be considered as a species that accumulates medium-chain saturated fatty acids (C12) and long-chain unsaturated fatty acids (C18). In conclusion, many of its health benefits may be due to its high content of lauric acid. Therefore, this oil is highly regarded for its nutritional and health properties.

Keywords: fatty acids; fruits; Lauraceae; lauric acid; Ocotea caudata

Resumen

En la familia de las Lauraceae el género Ocotea presenta especies como fuentes de madera para la construcción, la ebanistería, el biocombustible, la desinfección, la cosmética y para el tratamiento de diversas enfermedades. Ocotea caudata tiene pocos estudios fitoquímicos y farmacognósticos: este trabajo es el primero que estudia los frutos de esta especie. Los frutos de O. caudata (Nees) Mez de Colombia fueron investigados por su contenido de aceite. La extracción con heptano del aceite de los frutos de O. caudata arrojó un 54.9 % de lípidos totales y se determinó la composición de ácidos grasos. El análisis GC-FID (Gas Chromatography Flame Ionization Detector) del aceite indicó que el ácido láurico es el ácido graso más abundante en el aceite (51.7 %, C12:0). Además del ácido láurico, se encontró ácido oleico (23.6 %, C18:1) y ácido palmítico (16.6 %, C16:0). El ácido láurico se considera fuente de numerosos beneficios para la salud. Por lo tanto, el alto contenido de ácido láurico en O. caudata ofrece la posibilidad de utilizar este aceite como componente importante en usos nutricionales y de salud. O. caudata puede considerarse una especie que acumula ácidos grasos saturados de cadena media (C12) y ácidos grasos insaturados de cadena larga (C18).

Palabras claves: ácidos grasos; frutos; Lauraceae; ácido laurico; Ocotea caudata

Introduction

Lauraceae is one of the most important families of angiosperms and includes 55 genera and over 2000 species (Chanderbali et al., 2001; Schmid, 2005; van der Werff and Richter, 1996). Seed oil from the Lauraceae family is characterized by its high lauric acid content, whose concentration can vary depending on the species (Badami and Patil, 1980; Hopkins et al., 1966). This fatty acid is unusual in plants and is highly valued for its health and nutritional benefits. In traditional medicine, lauric acid is used for the treatment of viral infections, such as the common cold, the flu, genital herpes, and many other conditions (WebMed, 2023). Recent studies show that the consumption of lauric acid may be related to a decrease in cognitive impairment (Currenti et al., 2023). The therapeutic properties attributed to lauric acid are its anticancer, antibacterial (against Streptococcus aureus), antiviral (against herpes simplex, vesicular stomatitis and visna), and antifungal (against Candida albicans) activity (Sandhya et al., 2016; Nitbani et al., 2022). Some studies propose that lauric acid helps lower cholesterol levels, combat cardiovascular diseases, and decrease body weight (de Roos et al., 2001; Sandhya et al., 2016).

To date, lauric acid has been found in most Lindera, Cinnamomum, Laurus, Umbellularia and Litsea species. In contrast, capric acid has been mainly described in Sassafras albidum (Lauraceae) seed fat (Badami and Patil, 1980; Gottlieb, 1972; Hopkins et al., 1966; Litchfield, 1971). Lastly, in Persea americana (Lauraceae), oleic, palmitic, and linoleic acids have been observed (Ozdemir and Topuz, 2004).

Ocotea is one of the largest pantropical genera in the Lauraceae family, which comprises approximately 350 species distributed primarily in the Neotropics of America, and some species have also been found in Micronesia, Madagascar and African countries (Rakotondraibe et al., 2015; van der Werff, 2002). Phytochemical studies on the genus Ocotea have reported the presence of aporphine and benzylisoquinoline alkaloids, alkylphenols, arylpropenes, benzopyrans, butanolides, coumarins, essential oils, esters, flavonoids, lignans, neolignanes, sesquiterpenes, steroids, and saponins. Among the biological activities reported are antiherpetic, antioxidant, antimicrobial, antiplasmodial, antimalarial, antifungal, anti-inflammatory, antiproliferative, antithrombotic, antinociceptive, antiprotozoal, antileishmanial, and molluscicidal. Furthermore, it can be employed in the treatment of cardiovascular diseases. It is also known for its cytotoxicity, its larvicidal and cruzain inhibitory activity, and as a mutagenic and recombinogenic agent leading to lethality and toxicity (Garcez et al., 2011; Monte Neto et al., 2008; Montrucchio et al., 2012; Salleh and Ahmad, 2017; da Silva et al., 2017; Zhou et al., 2000). Moreover, in some regions of Amazonia, this oil is used as biofuel to replace kerosene (Marques et al., 2012).

Ocotea caudata (Nees) Mez. is known by the basionyms Ocotea cernua (Nees) Mez. and Oreodaphne cernua Nees (van der Werff, 2002). In Colombia, it is known as jigua negro, jigua baboso, amarillo and laurel. In Peru, it has several common names such as moena, moena blanca, roble, roble blanco and puchuguero, and in Brazil as lauro-preto. The wood is semi-hard, which makes it workable and durable, and highly prized for carpentry and joinery (Reynel and Rodriguez, 2006). In these South American countries, the wood of this plant is greatly valued for its quality and its use as a living fence. To the best of our knowledge, there are few phytochemical and pharmacognostic studies on this plant species; therefore, the present study is the first contribution on fatty acid content in Ocotea caudata (Nees) Mez fruits from Colombia. In addition, considering the large number of new sources of lauric acid with interesting nutritional, cosmetic and pharmaceutical uses, this research aims to contribute to exploring the potential of non- traditional oleaginous plants available in Colombia and to support the chemotaxonomy of the Lauraceae family.

Materials and methods

Plant material sampling

Ocotea caudata (Ness) Mez mature fruits were randomly collected from five to eight plants in the municipality of Puerto López, Meta, Colombia [4°5’28” N, 73°04’21” W]. Fruit samples were separated, cleaned, freeze-dried (-80 °C), grounded and stored in the dark at -20 °C until analysis. The collected specimens were identified by Professor Adolfo Jara Muñoz at the Universidad Nacional de Colombia. The voucher specimen (COL544562) was deposited in the Herbario Nacional Colombiano, in Colombia.

Oil extraction and preparation of fatty acid methyl esters (FAME)

The powdered material (27.3 g) was extracted in a Soxhlet equipment using heptane (500 mL) as solvent. The extraction was carried out according to the AOAC method (AOAC, 2012), with modifications. Briefly, the sample was processed for 4 h at the heptane’s boiling point for 20 runs. The solvent was removed using a rotary vacuum evaporator at 40 °C (Heidolph Instruments, Hei-Vap Precision). The oil fraction was then transferred to sealed amber glass bottles, which were capped and stored at -20 °C until analysis.

O. caudata lipid fraction derivatization was carried out according to Metcalfe et al. (1966) as follows: 50 mg of the lipid fraction was placed in a test tube and 5 mL of BF3-MeOH complex at 14 % were added. Then, the mixture was heated at 85 °C for one hour.

Finally, FAME were extracted with heptane (1 mL x 3), dried with anhydrous Na2SO4, and the resulting dried solution was injected into the gas chromatograph for analysis. All experiments were carried out in triplicate (Metcalfe et al., 1966; Parimelazhagan, 2016).

Gas chromatography with flame ionization detection (GC-FID) analysis

The FAME content was quantified using a Shimadzu GC-2014 gas chromatograph-FID equipped with a RT- 2560 (100 m x 250 μm x 0.2 μm) column and an AOC- 20i+s autosampler under the following conditions: 450 mL min-1 air pressure and 45 mL min-1 H2 pressure.

Helium was used as the carrier gas at 1.2 mL min-1 with a split of 1:200, and a 1.0 mL sample injection. The oven temperature was maintained at 100 °C for 3 min, then increased to 3 °C min-1 and held at a final temperature of 240 °C for 10 min. Injector and flame ionization detector (FID) temperatures were 225 °C and 240 °C, respectively. Identification and quantification of FAME by FID response integration were accomplished by comparing the relative retention times of the sample peaks with those of authentic standards (Food Industry FAME Mix # 35077, Restek, 30 mg mL-1 CH2Cl2).

Fatty acid methyl ester quantification was carried out by the external calibration method. Calibration curves were prepared by diluting a known weight of a standard FAME in CH2Cl2 and analyzing it in the concentration range (Dodds et al., 2005). For quantitative purposes, they were prepared in triplicate. For each FAME, the integrated peak area versus the amount of standard methyl ester were plotted.

Gas chromatography-mass spectrometry (GC-MS) analysis

FAME content identification was confirmed using a gas chromatograph Agilent 7890A GC with split/ splitless injector coupled to a Hewlett Packard 5973 mass selective detector, equipped with a HP- FFAP (50 m x 200 μm x 0.33 μm) column. Operating conditions were as follows: it was used as the carrier gas helium with a 1.0 mL min-1 flow. The oven temperature program was set at 100 °C (0 min) to 240 °C (10 min) at 5 °C min-1 with an injection temperature of 250 °C, and a detector temperature of 285 °C. Ionization voltage was set at 70 eV with a 60 μA ionization current; the range acquisition mass was 40-450 m z-1 with a split of 1:30. The compounds were identified by the comparison of their mass spectra with those of NIST08, Wiley9L libraries spectra. The integration of the total chromatogram, expressed as percent area, has been used as a rough parameter to ascertain the relative composition of the lipid fraction (Dodds et al., 2005).

Statistical analysis

The results are representative of three independent replicates and are expressed as mean ± SD. Regression and correlation analyses were determined using SPSS version 21.0 (Chicago, IL).

Results and discussion

O. caudata heptane oil extraction from the fruit yielded a 15 g lipid fraction after the solvent was evaporated under reduced pressure, which represents 54.9 % of the total lipids. Studies on the extraction of fatty acids from Lauraceae species’ seeds using petroleum ether as the extraction solvent results in 20 % and 60 % oil (Hopkins et al., 1966; Kotoky et al., 2007; Wang et al., 1985). Fatty acid extraction yields using petroleum ether and hexane are comparable; however, better yields have been reported with hexane (Taha et al., 1988). Furthermore, heptane is an alternative to hexane for fatty acid extraction; the yield and quality of some seed oils obtained with heptane are similar to extraction processes with hexane (McConnell and Farag, 2013; Taha et al., 1988). In the present work, heptane was selected as the extraction solvent since oil extraction with heptane is faster in comparison with hexane. Moreover, it is more environmentally friendly and less toxic than hexane (McConnell and Farag, 2013; Taha et al., 1988).

Linearity response and sensitivity

Table 1 presents the calibration curves for the different FAME selected including their retention time (Rt), and equation and correlation coefficients (r). Fatty acid standards were detected by GC-FID as FAME in approximately 60 min of analysis. According to the linear regression equations obtained from the calibration curves, the detector response to the tested concentrations was linear with the coefficient of correlation values higher than 0.99 for all fatty acids studied. These values demonstrate that the calibration model was efficient for all standards assayed. For all fatty acids, the slope values of the regression line were similar (mean value of 6.68e-3), which indicates a comparable sensitivity for each fatty acid (Salimon et al., 2017).

Table 1 FAME standards estimated regression parameters obtained from calibration curves 

r = coefficient of correlation, a y = FAME peak area, X = FAME concentration.

GC-FID and GC-MS analysis

Analysis of the O. caudata seed oil obtained from the GC-FID heptane extract presented five prominent peaks (Figure 1). O. caudata concentration and prevalence of saturated, monounsaturated, and poly-unsaturated fatty acids is described in Table 2. Four saturated, one monounsaturated, and one polyunsaturated fatty acids were observed in this study. Lauric acid (C12:0, 51.7 %), oleic acid (C18:1, 23.6 %) and palmitic acid (C16:0, 16.6 %) were the main fatty acids identified. A discrete quantity of myristic acid (C14:0, 2.3 %), stearic acid (C18:0, 2.0 %) and linoleic acid (C18:2, 3.8 %) was also present in the species. The oil was rich in saturated fatty acids, particularly lauric acid, which agrees with the report for other Lauraceae seeds. Hopkins et al. (1966), studied the seed oils of six Lauraceae species (Lindera umbellata, Lindera praecox, Lindera benzoin, Laurus nobilis, Cinnamomum camphora, Umbellularia californica) and classified them into three types: The first grouped species contain saturated fatty acids (C10 and C12), to this group belong Lindera benzoin, Lindera Praecox, Cinnamomum camphora and Umbellularia californica. The second group encompasses principally saturated medium-chain acids (C12) and unsaturated long-chain acids (C18), as observed in Laurus nobilis. Lastly, the third group contains medium-chain saturated and unsaturated fatty acids (C10 - C14) found mainly in Lauraceae such as Lindera umbellata. Due to its contents of lauric acid (51.7 %, C12:0) and oleic acid (23.6 %, C18: 2), O. caudata was classified in the second group.

Figure 1 O. caudata fruit lipid fraction from GC-FID chromatogram: Capric acid (22.94 min), lauric acid (27.48 min), tridecanoic acid (30.03 min), myristic acid 32.65 min), pentadecanoic acid (35.15 min), palmitic acid (37.52 min), stearic acid (41.94 min), oleic acid (43.35 min), linoleic acid (45.31 min), and heneicosanoic acid (48.81 min). 

Table 2 Fatty acid composition percentage in O. caudata fruits 

Note: Values are presented as means of total fatty acid methyl ester percentage (n = 3). SD = standard deviation.

The amount of lauric acid found in the species is similar to that of coconut oil (50 %) (Nitbani et al., 2022), suggesting its suitability for soap, detergent and cosmetic production, and all products based on lauric acid, as well as their use as an edible oil (Liebert, 1987). Furthermore, lauric acid inhibits bacteria growth, such as Salmonella sp., Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Bacillus stearothermophilus, and others of the Candida, Micrococcus, Pneumococcus, and Pseudomonas genera (Barlina et al., 2022).

Lauric acid has clinical significance, it is the main antiviral and antibacterial substance found in human breast milk, esterified into the monoglyceride form, known as monolaurin (Lieberman et al., 2006; Sandhya et al., 2016). Lauric acid antiviral and antibacterial activity is greater than other medium- chain glycerides, such as caprylic acid (C8:0), capric acid (C10:0) or myristic acid (C14:0) glycerides (Walters et al., 2003; Sandhya et al., 2016). However, it is still unclear whether monolaurin can be synthesized from lauric acid in humans. Nevertheless, there is evidence that some is formed (Lieberman et al., 2006). Monolaurin is biologically many more times active than lauric acid against viruses, coronaviruses and bacteria, posing the interesting question on its conversion rate in humans (Lieberman et al., 2006; Subroto et al., 2020; Nitbani et al., 2022). Hence, it is necessary to study these aspects in O. caudata for economic and commercial use.

Conclusions

The present work, though preliminary in nature, revealed that fruits of Ocotea caudata might be explored for the extraction of lauric acid, which is used in the manufacture of industrial and pharmaceutical products. The study of fatty acids in fruits of Lauraceae contributes to the chemotaxonomy of the family according to their composition of fatty acids, classifying Ocotea caudata within the species that accumulate medium-chain saturated fatty acids (C12) and long-chain unsaturated fatty acids (C18) in their seeds.

References

AOAC (2012). 948.22-1948, Fat (crude) in nuts and nut products. Gravimet. In AOAC (Ed.), Official Methods of Analysis, 19th ed. AOAC International. http://www.aoacofficialmethod.org/index.php?main_page=product_info&products_id=588Links ]

Badami, R. C. and Patil, K. B. (1980). Structure and occurrence of unusual fatty acids in minor seed oils. Progress in Lipid Research, 19(3-4), 119-153. https://doi.org/10.1016/0163-7827(80)90002-8Links ]

Barlina, R.; Dewandari, K. T.; Mulyawanti, I. and Herawan, T. (2022). Chapter 30 - Chemistry and composition of coconut oil and its biological activities. In Mariod, A. A. (Ed.), Multiple Biological Activities of Unconventional Seed Oils (pp. 452). Academic Press. https://doi.org/10.1016/B978-0-12-824135-6.00025-8Links ]

Chanderbali, A. S.; van der Werff, H. and Renner, S. S. (2001). Phylogeny and historical biogeography of Lauraceae: Evidence from the chloroplast and nuclear genomes. Annals of the Missouri Botanical Garden, 88(1), 104-134. https://doi.org/10.2307/2666133Links ]

Currenti, W.; Godos, J.; Alanazi, A. M.; Lanza, G.; Ferri, F.; Caraci, F.; Grosso, G.; Galvano, F. and Castellano, S. (2023). Dietary fats and cognitive status in Italian middle-old adults. Nutrients, 15(6), 1429. https://doi.org/10.3390/nu15061429Links ]

Da Silva, J. K.; da Trindade, R.; Moreira, E. C.; Maia, J. G. S.; Dosoky, N. S.; Miller, R. S.; Cseke, L. J. and Setzer, W. N. (2017). Chemical diversity, biological activity, and genetic aspects of three Ocotea species from the Amazon. International Journal of Molecular Sciences, 18(5), 1081. https://doi.org/10.3390/ijms18051081Links ]

De Roos, N. M.; Schouten, E. G. and Katan, M. B. (2001). Consumption of a solid fat rich in lauric acid results in a more favorable serum lipid profile in healthy men and women than consumption of a solid fat rich in trans-fatty acids. The Journal of Nutrition, 131(2), 242-245. https://doi.org/10.1093/jn/131.2.242Links ]

Dodds, E. D.; McCoy, M. R.; Rea, L. D. and Kennish, J. M. (2005). Gas chromatographic quantification of fatty acid methyl esters: Flame ionization detection vs. electron impact mass spectrometry. Lipids, 40(4), 419-428. https://doi.org/10.1007/s11745-006-1399-8Links ]

Garcez, F. R.; da Silva, A. G. F.; Garcez, W. S.; Linck, G.; Matos, M. C. F.; Santos, E. C. and Queiroz, L. M. M. (2011). Cytotoxic aporphine alkaloids from Ocotea acutifolia. Planta Medica, 77(4), 383-387. https://doi.org/10.1055/s-0030-1250401Links ]

Gottlieb, O. R. (1972). Chemosystematics of the lauraceae. Phytochemistry, 11(5), 1537-1570. https://doi.org/10.1016/0031-9422(72)85001-5Links ]

Hopkins, C. Y.; Chisholm, M. J. and Prince, L. (1966). Fatty acids of Lindera umbellata and other Lauraceae seed oils. Lipids , 1(2), 118-122. https://doi.org/10.1007/BF02533002Links ]

Kotoky, R.; Pathak, M. G. and Kanjilal, P. B. (2007). Physico-chemical characteristics of seed oils of some Litsea species found in North-East India. Natural Product Radiance, 6(4), 297-300. https://nopr.niscpr.res.in/bitstream/123456789/7874/1/NPR6%284%29297-300.pdfLinks ]

Lieberman, S.; Enig, M. G. and Preuss, H. G. (2006). A review of monolaurin and lauric ccid: Natural virucidal and bactericidal agents. Alternative and Complementary Therapies, 12(6),310-314. https://doi.org/10.1089/act.2006.12.310Links ]

Liebert, M. A. (1987). 3. Final report on the safety assessment of oleic acid, lauric acid, palmitic acid, myristic acid, and stearic acid. Journal of the American College of Toxicology, 6(3), 321-401. https://doi.org/10.3109/10915818709098563Links ]

Litchfield, C. (1971). Positional distribution of medium chain length fatty acids in dicotyledon seed triglycerides. Chemistry and Physics of Lipids , 6(2), 200-204. https://doi.org/10.1016/0009-3084(71)90042-9Links ]

Marques, C. A. (2012). Importância econômica da família Lauraceae Lindl. Floresta e Ambiente, 8(1), 195-206. http://www.floram.org/article/588e21fae710ab87018b45cfLinks ]

McConnell, B. O. and Farag, I. H. (2013). Kinetics study of the solvent extraction of lipids from Chlorella vulgaris. International Journal of Engineering and Technical Research (IJETR), 1(10), 28-37. https://www.erpublication.org/published_paper/IJETR012016.pdfLinks ]

Metcalfe, L. D.; Schmitz, A. A. and Pelka, J. R. (1966). Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analytical Chemistry, 38(3), 514-515. https://doi.org/10.1021/ac60235a044Links ]

Monte Neto, R. L.; Sousa, M. A.; Dias, C. S.; Barbosa Filho, J. M. and Oliveira, M. R. (2008). Yangambin cytotoxicity: A pharmacologically active lignan obtained from Ocotea duckei Vattimo (Lauraceae). Zeitschrift Für Naturforschung C, 63(9-10), 681-686. https://doi.org/10.1515/znc-2008-9-1012Links ]

Montrucchio, D. P.; Miguel, O. G.; Zanin, S. M. W.; da Silva, G. A.; Cardozo, A. M. und Santos, A. R. S. (2012). Antinociceptive effects of a chloroform extract and the alkaloid dicentrine isolated from fruits of Ocotea puberula. Planta Medica , 78(14), 1543-1548. https://doi.org/10.1055/s-0032-1315026Links ]

Nitbani, F. O.; Tjitda, P. J. P.; Nitti, F.; Jumina, J. and Detha, A. I. R. (2022). Antimicrobial properties of lauric acid and monolaurin in virgin coconut oil: A review. ChemBioEng Reviews, 9(5), 442- 461. https://doi.org/10.1002/cben.202100050Links ]

Ozdemir, F. and Topuz, A. (2004). Changes in dry matter, oil content and fatty acids composition of avocado during harvesting time and post-harvesting ripening period. Food Chemistry, 86(1), 79-83. https://doi.org/10.1016/j.foodchem.2003.08.012Links ]

Parimelazhagan, T. (2016). Pharmacological Assays of Plant-Based Natural Products (vol. 71). Springer International Publishing. https://doi.org/10.1007/978-3-319-26811-8Links ]

Rakotondraibe, L. H.; Graupner, P. R.; Xiong, Q.; Olson, M.; Wiley, J. D.; Krai, P.; Brodie, P. J.; Callmander, M. W.; Rakotobe, E.; Ratovoson, F.; Rasamison, V. E.; Cassera, M. B.; Hahn, D. R.; Kingston, D. G. I. and Fotso, S. (2015). Neolignans and other metabolites from Ocotea cymosa from the Madagascar rain forest and their biological activities. Journal of Natural Products, 78(3), 431-440. https://doi.org/10.1021/np5008153Links ]

Rodriguez, C. R. (2006). Árboles útiles del Ande Peruano: una guía de identificación, ecología y propagación de las especies de la Sierra y los Bosques Montanos en el Perú. Tarea Gráfica Educativa. [ Links ]

Salimon, J.; Omar, T. A. and Salih, N. (2017). An accurate and reliable method for identification and quantification of fatty acids and trans fatty acids in food fats samples using gas chromatography. Arabian Journal of Chemistry, 10(supplement 2), S1875-S1882. https://doi.org/10.1016/j.arabjc.2013.07.016Links ]

Salleh, W. M. N. H. W. and Ahmad, F. (2017). Phytochemistry and biological activities of the genus Ocotea (Lauraceae): A review on recent research results (2000-2016). Journal of Applied Pharmaceutical Science, 7(5), 204-218. https://doi.org/10.7324/JAPS.2017.70534Links ]

Sandhya, S.; Talukdar, J. and Bhaishya, D. (2016). Chemical and biological properties of lauric acid: A review. International Journal of Advanced Research (IJAR), 4(7), 1123-1128. https://doi.org/10.21474/IJAR01Links ]

Schmid, R. (2005). Review of Flowering Plants of the Neotropics, by Smith, N., Mori, S. A., Henderson, A., Stevenson, D. W. and Heald, S. V. Taxon, 54(1), 260. https://doi.org/10.2307/25065340Links ]

Subroto, E. and Indiarto, R. (2020). Bioactive monolaurin as an antimicrobial and its potential to improve the immune system and against COVID-19: A review. Food Research, 4(6), 2355-2365. https://doi.org/10.26656/fr.2017.4(6).324Links ]

Taha, F. S.; Helmy, H. E. and El-Nockrashy, A. S. (1988). Changes in cottonseed oil when used for frying vegetable products containing chlorophyll. Journal of the American Oil Chemists’ Society, 65(2), 267-271. https://doi.org/10.1007/BF02636414Links ]

Van der Werff, H. (2002). A synopsis of Ocotea (Lauraceae) in Central America and Southern Mexico. Annals of the Missouri Botanical Garden , 89(3), 429-451. https://doi.org/10.2307/3298602Links ]

Van der Werff, H. and Richter, H. G. (1996). Toward an improved classification of Lauraceae. Annals of the Missouri Botanical Garden , 83(3), 409-418. https://doi.org/10.2307/2399870Links ]

Walters, D. R.; Walker, R. L. and Walker, K. C. (2003). Lauric acid exhibits antifungal activity against plant pathogenic fungi. Journal of Phytopathology, 151(4), 228-230. https://doi.org/10.1046/j.1439-0434.2003.00713.xLinks ]

Wang, J. P.; Meng, S. J. and Li, J. M. (1985). Studies on the distribution of fatty acids in the oils of the family Lauraceae. Journal of Integrative Plant Biology, 27(2), 177-185. https://www.jipb.net/EN/Y1985/V27/I2/Links ]

WebMed. (2023). Vitamins & Supplements: Lauric acid - Uses, side effects, and more. In: In: https://www.webmd.com/vitamins/ai/ingredientmono-1138/lauric-acid (acces May 11, 2023). [ Links ]

Zhou, B.-N.; Johnson, R. K.; Mattern, M. R.; Wang, X.; Hecht, S. M.; Beck, H. T.; Ortiz, A. and Kingston, D. G. I. (2000). Isolation and biochemical characterization of a new topoisomerase I inhibitor from Ocotea leucoxylon. Journal of Natural Products , 63(2), 217-221. https://doi.org/10.1021/np990442sLinks ]

Received: November 03, 2022; Accepted: May 03, 2023

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