Introduction:

Gastric cancer in Nariño, Colombia, is a significant public health issue due to its high incidence and mortality. The eradication of Helicobacter pylori (H. pylori) is considered a crucial preventive strategy, but the failure of conventional treatments has driven the search for alternatives. Natural products, such as Cannabis sativa L. (C. sativa) extract, are being evaluated for their therapeutic potential.

Objective:

To evaluate the in vitro inhibitory activity of C. sativa extract, rich in cannabidiol, against the growth of H. pylori isolated from patients with gastric disease in Nariño, Colombia.

Methodology:

H. pylori was isolated and purified from gastric biopsies of patients from the Department of Nariño, Colombia. The C. sativa extract was obtained through supercritical carbon dioxide (CO2) extraction. The inhibitory activity was assessed using the agar dilution method to determine the minimum inhibitory concentration (MIC).

Results:

The MIC of C. sativa extract against H. pylori ranged from 35 to 55 μg/mL, indicating significant efficacy in inhibiting the bacteria.

Discussion:

The C. sativa extract demonstrated a notable inhibitory effect on H. pylori, suggesting its potential as a therapeutic alternative to conventional treatments that have failed.

Conclusion:

Cannabidiol-rich C. sativa extract exhibits effective in vitro inhibitory activity against H. pylori in patients from Nariño.