SciELO - Scientific Electronic Library Online

 
vol.15 issue2Variations in water quality and morbidity during capture and post-capture of loricariids species being sold in Acacias (Meta) ColombiaA case report concerning diffuse large and small cell extranodal lymphoma in a dog author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


ORINOQUIA

On-line version ISSN 0121-3709

Abstract

CASTRO-MOLINA, Susan L. et al. Detección del Polimorfismo 1843 en el Gen Receptor de Ryanodina Mediante la Técnica de PCR-SSCP. Orinoquia [online]. 2011, vol.15, n.2, pp.192-200. ISSN 0121-3709.

Pig stress syndrome (PSS) is a genetic disease caused by a nucleotide mutation in the RYR-2 gene encoding the ryanodine receptor (RYR-1), considered an autosomal recessive condition. PSS, or malignant hyperthermia, is characterised by the lowering of meat quality and animal death, leading to a pale, smooth and exhudative (PSE) carcass. The syndrome is triggered in genetically-susceptible individuals either by anaesthetic agents, like halothane, or stress conditions, such as transport, crowding and mating. Information about this syndrome's incidence and presence in Colombia is currently quite scarce. This study was aimed at determining the presence of the RYR-I T-allele in a porcine population from the Universidad National de Colombia's Marengo farm to identify individuals which were more susceptible to the syndrome. A total of 50 pigs (27 males and 23 females) were selected at random. CC genotype frequency for males was 0.59, 0.37 for the CT genotype and 0.04 for the TT genotype; in females this was 0.65 for the CC genotype, 0.13 for CT and 0.22 for TT. A total of 31 (62 %) individuals were considered healthy (CC), 13 (26 %) were carriers (CT) and six (12 %) were susceptible (TT). C and T allele frequency was 0.75 and 0.25, respectively. Hardy-Weinberg equilibrium comparison tests revealed that the population was in genetic disequilibrium (p ≤ 0.05). The results did show the presence of the allele responsible for the syndrome; this is a very significant factor to be considered when establishing an appropriate animal breeding programme by using marker-assisted selection.

Keywords : Porcine stress; mutation; PCR-SSCP; ryanodine receptor gene.

        · abstract in English | Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License