Abstract

ROMERO, Lolymar et al. Production of pigments from Arthrospira maxima cultivated in photobioreactors. Rev. colomb. biotecnol [online]. 2017, vol.19, n.1, pp.108-114. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v19n1.59671.

The culture of the cyanobacteria Arthrospira maxima can be done in open systems and closed systems or photobioreactors. The objective of the present research was to evaluate the pigment production of Arthrospira maxima grown on two types of photobioreactors. Batch system culture with Zarrouk´s medium was carried out under controlled conditions in a helicoidal and cylindrical photobioreactors during 30 days. Pigments determination was carried out in exponential and stationary growth phases. Biomass was extracted with 90% acetone, and the liposoluble pigments were injected in a HPLC. For the phycobiliprotein pigments extractions, four methods were tested: 1.- phosphate regulator/enzyme, 2.- alkaline solution, after treatment with CaCl2; 3.- phosphate buffer, previous treatment with dry ice, and 4.- water (4 °C), and subsequent determination by UV-Visible Spectrophotometry. The highest pigments content was obtained in the helicoidal photobioreactor cultures during the exponential phase (chlorophyll a 11.08±0.006 μg mL-1, β-carotene 1.82±0.003 μg mL-1, zeaxanthin 0.72±0.002 μg mL-1). Moreover, the highest contents of phycobilipretein pigments were obtained in a cylindrical photobioreactor, during the stationary phase, with phosphate buffer with dry ice extraction. Among the phycobiliproteins, the phycocyanin was in highest content (77.74±0.767 mg L-1), followed by allophycocyanin and phycoerythrin. It is concluded that the biomass of Arthrospira maxima presents biotechnological potential due to its high pigment contents.

Keywords : Pigments; Arthrospira maxima; photobioreactors.

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