Abstract

PENA, CAROLINA  and  ARANGO, RAFAEL. EVALUATION OF ETHANOL PRODUCTION USING RECOMBINANT STRAINS OF Saccharomyces cerevisiae FROM SUGAR CANE MOLASSES. Dyna rev.fac.nac.minas [online]. 2009, vol.76, n.159, pp.153-161. ISSN 0012-7353.

The Ethanol production, cellular growth and substrate consumption of three Saccharomyces cerevisiae strains: CBS8066 (control) and two recombinant CIB, GG570-CIBI and GG570-CIBII were evaluated under the effect of two sucrose concentrations (170 and 250g/L) and two substrates: industrial with sugar cane molasses and synthetic with sucrose. During the fermentative process on industrial substrate the major ethanol production was obtained at a sucrose concentration of 250g/L. Under these conditions, strain GG570-CIBII produced greater ethanol concentrations than control strain, reaching an average of 2,34 g/L, in addition, at 10h of fermentation, GG570-CIBII strain produced 8,02 g/L ethanol above control strain. On the other hand, strain GG570-CIBI produced 3,46 g/L ethanol less than control strain. In this way, strain GG570-CIBII cultivated on sugar cane molasses at a sucrose concentration of 250 g/L was tolerant to high sucrose concentration, and besides, this strain was capable of producing greater ethanol concentrations than control strain.

Keywords : recombinant Saccharomyces cerevisiae; pdc gene; adhII gene; Zymomonas mobilis; ethanol.

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