Abstract

QUINTERO MORENO, MÓNICA; MONTOYA CAMPUZANO, OLGA INÉS  and  GUTIERREZ SANCHEZ, PABLO A.. PURIFICATION AND CHARACTERIZATION OF A a-AMYLASE PRODUCED BY Bacillus sp. BBM1. Dyna rev.fac.nac.minas [online]. 2010, vol.77, n.162, pp.31-38. ISSN 0012-7353.

A novel a-amylase producing strain, Bacillus sp. BBM1, was isolated a soil sample from Universidad Nacional de Colombia sede Medellín. Morphological, Biochemical and molecular data suggests that this strain belongs to either B. subtillis or B. amyloliquefaciens species. a-amylase BBM1 was purified by ammonium sulphate precipitation and its molecular weight estimated as 77.7 kDa by SDS-PAGE electrophoresis. This enzyme was completely stable at 60°C as presents significant activity in the 30-80°C range. Amylase BBM1 has an optimal pH in the range 5.0-7.0 and does not require calcium. All this properties make this enzyme interesting for future industrial applications.

Keywords : Bacillus; enzymes; a-amylase.

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