Abstract

ESTUPINAN DURAN, HUGO ARMANDO et al. ADSORPTION STUDY OF PROTEINS ON SURFACES OF POLY-LACTIC ACID BY USING ELECTROCHEMICAL AND MICROGRAVIMETRIC TECHNIQUES. Dyna rev.fac.nac.minas [online]. 2011, vol.78, n.169, pp.167-175. ISSN 0012-7353.

Electrochemical methods were used to promote and evaluate the oriented adsorption of albumin and collagen on polymer surfaces of polylactic acid by applying an anodic overpotential in the range of 0 V to 0.45 V. The adsorption was studied using simultaneous measurements of electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) in a temperature range of 25°C to 45°C, and protein variations between 5 and 20 mg/ml phosphate buffer solution of pH 7,4. Increasing protein concentration in the electrolyte reduces resistance to charge transfer associated with the interaction between protein and electrode, thus showing a greater adsorption on the surface, confirmed by the increased mass measured by QCM. With the electrochemical polarization, the hydrophilicity of PLA coating was improved, showing a higher affinity and adsorption capacity with collagen-rich solutions.

Keywords : Adsorption; bovine serum albumin; collagen; polymers biodegradables; gravimetric; electrochemical.

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