Abstract

TRUJILLO, CARLOS; RESTREPO, JOHN F.  and  GARCIA-SUCERQUIA, JORGE. VIDEO RATE RECONSTRUCTION OF HOLOGRAMS FROM DIGITAL IN-LINE HOLOGRAPHIC MICROSCOPY. Dyna rev.fac.nac.minas [online]. 2012, vol.79, n.173, pp.25-31. ISSN 0012-7353.

Digital in-line holographic microscopy (DIHM) is perhaps the simplest methodology to obtain three-dimensional information about the micrometer world: a point source and a digital recording device is the hardware needed. In this microscopy technique, the recovery of the complex amplitude scattered by the specimens, relies on quite robust algorithms operating on two-dimensional intensity recordings; each recording is formed by millions of pixels. The elapsed time for carrying out such information processing has concealed the wider application of DIHM. In this paper, by the use of the power provided by the graphics processing units (GPU) along with a reduced number of operations, a methodology for numerical reconstruction at video rate of DIHM holograms is presented. This strategy allows for reconstructing 1 megapixel DIHM holograms up to 32 frames per second with no change in spatial resolution performance of this lensless microscopy architecture. The experimental results shown on this paper exhibit the three-dimensional capabilities of DIHM operating at video rate: a hologram taken from a two-dimensional self-organised monolayer of micrometer sized beads is reconstructed a video rate.

Keywords : digital in-line holographic microscopy; graphics processing units; three-dimensional microscopy; video rate numerical reconstruction.

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