Resumo

ARANGO, GLORIA et al. LASSER DIFFRACTION PATTERNS AND IMAGE ANALYSIS TO DIFFERENTIATE THREE TYPES OF BACTERIAL COLONY. Dyna rev.fac.nac.minas [online]. 2013, vol.80, n.177, pp.69-74. ISSN 0012-7353.

Fast and accurate bacterial colony identification is a common requirement in many fields, and usually is supported by the use microscopic, biochemical and molecular tools. But procedures involving these tools might be expensive or time consuming. This study uses image analysis of laser diffraction patterns, obtained from intact bacterial colonies in order to asses its suitability to differentiate bacterial colonies. We also tested the effect of the colony incubation period on these patterns, and the role of the volume of culture media on the laser light diffraction patterns. Laser diffraction patterns were obtained from the five bacterial isolates B1, B2, B3, B2Ca y B2Cb. Bacterial colony differentiation by its diffraction patterns was assessed using isolates B2, B3, B2Ca. The role of colony incubation period on obtained diffraction patterns was tested by using isolates B1 and B2Cb, whereas agar plates without colonies were used to assess the role of different volumes of culture media on its laser diffraction patterns. It was found that the volume of culture media may reduce the quality of the laser diffraction patterns. A multivariate dimensional analysis on laser diffraction patterns also indicated that these can be used to differentiate the bacterial colonies included in the test. Finally, the length of the incubation period on the differences among diffraction patterns of the same isolate appears to be negligible. It is concluded that this simple technique could be used to support the design of sensible equipments based mainly on cheap, locally available supplies, which in turn might support industry and research needs requiring fast and accurate bacterial colony detection.

Palavras-chave : laser diffraction; biodiversity; ImageJ; soil microorganisms.

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