Resumo

NOVA-LOPEZ, Carlos Julio et al. Expression of recombinant Cry 1Ac protein in potato plant cell suspension culture: Establishment of culture and optimization of biomass and protein production by nitrogen supply. Dyna rev.fac.nac.minas [online]. 2017, vol.84, n.201, pp.34-41. ISSN 0012-7353.  https://doi.org/10.15446/dyna.v84n201.59829.

Plant cell suspension cultures have been proposed as alternative platforms for the expression of recombinant proteins with therapeutic application because of its potential advantages over traditional bacterial and mammalian cells-based platforms. In this work, a protocol for the establishment of potato suspensions (S. tuberosum) genetically modified with Cry 1Ac gene was obtained and the kinetics of biomass and recombinant protein production were characterized. Internodal explants and MS medium supplemented with 2.0 mg L-1 of 2,4- D showed the best response in terms of callus formation. The maximum specific growth rate for suspensions was 0.12 d-1, with a maximum biomass concentration of 1,41 g L-1 at the end of exponential phase. This biomass concentration was improved to 3.94 g L-1 by doubling the concentration of NO3- and NH4+ in the culture medium.

Palavras-chave : Plant cell suspension culture; recombinant proteins; Solanum tuberosum.

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