Abstract

ESPINOSA, Claudia et al. Evaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes. Dyna rev.fac.nac.minas [online]. 2018, vol.85, n.205, pp.348-354. ISSN 0012-7353.  https://doi.org/10.15446/dyna.v85n205.68767.

Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.

Keywords : carbon nanotubes; genotoxicity; mutagenicity tests; lymphocytes.

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