Abstract

MORENO, Daniel; VARGAS, Fabio  and  LOPEZ, Esperanza. Effect of the liquid-to-powder ratio on physicochemical properties of calcium phosphate cements and of these properties over Biofilm thickness of adhered Staphylococcus Aureus. Dyna rev.fac.nac.minas [online]. 2021, vol.88, n.219, pp.102-110.  Epub Mar 15, 2022. ISSN 0012-7353.  https://doi.org/10.15446/dyna.v88n219.93812.

Tricalcium phosphate (TCP) synthetized by high temperature solid state reaction at 1400°C and three derived calcium phosphate cements (CPCs) prepared at liquid to powder (L/P) ratios of 0.33, 0.44 and 0.55 ml/g, respectively, were physicochemically characterized. Calcium deficient hydroxyapatite crystals were identified by scanning electron microscopy on CPC, and differences in crystal sizes were observed at different L/P ratios. Also, the biofilm thickness of two Staphylococcus Aureus (S.aureus) strains grown for 24 hours on the three CPC are reported. A dependence of the biofilm thickness with the specific surface area (SSA) of CPC was identified. They are directly proportional for non-extracellular polysaccharide substances (EPS) producing S.aureus and inversely proportional for EPS producing S.aureus. Non-proportional behavior between the SSA and mechanical strength of the CPC was observed as L/P ratio increases.

Keywords : calcium phosphate cement; tricalcium phosphate; Staphylococcus aureus; biofilm thickness; specific surface area; Extracellular polysaccharide substances.

        · abstract in Spanish     · text in English     · English ( pdf )