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Revista Colombiana de Ciencias Químico - Farmacéuticas

Print version ISSN 0034-7418On-line version ISSN 1909-6356

Abstract

MORANTES, Marcela et al. Development and validation of an analytical method by HPLC for quantification of phenobarbital in extemporaneous suspensions. Rev. colomb. cienc. quim. farm. [online]. 2010, vol.39, n.1, pp.68-78. ISSN 0034-7418.

In this article we present the data obtained during the development, standardization and validation of a high performance liquid chromatography method for the quantification of phenobarbital in an extemporaneous suspension using butobarbital as internal standard. The chromatographic method uses a UV detector with diode array (DAD) as detection system, as mobile phase a mixture of H3PO4 0.1% in methanol and H3PO4 to 0.1% in water (48:52) and column chromatography C18 stabilized at 35 °C. The validated methodology was selective, linear, precise and accurate. The linearity was evaluated for concentrations between 4.8 and 24 mcg/mL. The developed methodology was used to carry out the study of stability of an extemporaneous suspension of phenobarbital used in the Clinic Country for the treatment of convulsions in pediatric patients.

Keywords : phenobarbital; extemporaneous suspension; validation; HPLC; diode array.

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