Revista Colombiana de Ciencias Químico - Farmacéuticas
Print version ISSN 0034-7418
ESCOBAR M, Linamaría; MORANTES, Sandra; CORDERO, Claudia P. and ARISTIZABAL, Fabio A.. Implementation of in vitro strategies to evaluate the functionality of a Colombian fetal bovine serum. Rev. colomb. cienc. quim. farm. [online]. 2011, vol.40, n.2, pp. 201-221. ISSN 0034-7418.
In this work, we present the results of biological tests performed to evaluate the functionality and performance of fetal bovine serum produced in Colombia, on the growth of cell cultures with different nutritional requirements. Performing cloning efficiency tests, plating efficiency and growth curves of cell lines and fibroblasts, showed that the Colombian serum can be used with a good performance, in continuous culture of cell lines A549, PC-3, HT-29, MDA-MB231, in fibroblasts LSPG8G cells, MDBK and hybridomes can be used in a minimum concentration of 10% and medium every 48 hours. It should be noted that none of the treatments showed a toxic or adverse effect on the growth of the cells used, although in some cases had less functionality compared to cell growth by comparing the results with other qualified serum. Therefore, the Colombian showed to be suitable for the propagation of short-term culture cells, as well as transitional procedures such as trypsin inactivation, and cryopreservation.
Keywords : cell culture; cell growth; bioassays; bovine serum.