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Revista Colombiana de Ciencias Químico - Farmacéuticas
versão impressa ISSN 0034-7418
Resumo
SIERRA, Noralba e GOMEZ, Gustavo. Validation of a high performance liquid chromatography method for the simultaneous determination of sodium piperacillin and sodium tazobactam in human injections. Rev. colomb. cienc. quim. farm. [online]. 2014, vol.43, n.1, pp.5-21. ISSN 0034-7418. https://doi.org/10.15446/rcciquifa.v43n1.45461.
A reverse phase high performance liquid chromatographic method was developed for the simultaneous quantitative assay of sodium piperaciline and sodium tazobactam. The method was validated and then applied to the quantitative determination assay of each of two substances A C18 column stabilized at 38 °C was used and the detection was performed at 220 nm. A mixture of acetonitrile and tetrabutylammonium hydroxide 0,005M pH 5,0 (31:69) was used as mobile phase. The method is selective, linear and shows a good repeatibility. The robustness was also verified. These properties besides their simplicities make it convenient for the objective proposed. The validated method was applied for the simultaneous assay of piperaciline and tazobactam in one market products with sanitary register at Instituto Nacional de Vigilancia y Control de Medicamentos y Alimentos (INVIMA).
Palavras-chave : Reverse high performance liquid chromatography; Sodium piperaciline; Sodium tazobactam; UV detection; Validation.