Resumo

SANABRIA, Lina M.; MARTINEZ, Jorge A.  e  BAENA, Yolima. Validation of an analytical HPLC-DAD methodology for the quantification of caffeine in an in vitro permeation test using porcine buccal mucosa. Rev. colomb. cienc. quim. farm. [online]. 2017, vol.46, n.2, pp.202-219. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v46n2.67956.

This paper presents the results of the development and validation of an analytical methodology through high performance liquid chromatography with a diode array detector (HPLC-DAD) for the quantification of caffeine, using a C18 reverse phase column with UV detection at 273 nm and a mobile phase consisting exclusively of water/acetonitrile (80:20 v/v). The evaluated performance characteristics allowed to verify that there is an adequate selectivity, a linearity between 0.5 and 50 /µg/mL, with precision expressed as RSD in less than 2%, a recovery rate of 99.9%, and limits of detection and quantification for the method of2.99 and 2.69 ng/mL respectively. The proposed method is useful for determining the oral permeation profile of caffeine by an in vitro study with Franz cells using porcine buccal membrane.

Palavras-chave : Validation; caffeine; HPLC; permeability; buccal mucosa; Franz cells.

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