Abstract

MARTINEZ, Laura A.; MARTINEZ, Jorge A.  and  BAENA, Yolima. Validation of an analytical methodology by HPLC-DAD for the quantification of complexed benzoic acid, in an in vitro transdermal permeation assay using pig skin. Rev. colomb. cienc. quim. farm. [online]. 2018, vol.47, n.3, pp.424-440. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v47n3.77374.

This paper presents the studies carried out about the validation of an analytical methodology by high performance liquid chromatography with diode array detector (HPLC-DAD) for the quantification of benzoic acid in polyelectrolyte complexes obtained with Eudragit® E100. Performance characteristics of the methodology were evaluated, finding that this is selective; linear in the concentration range of 2 to 10 (g / mL; accurate with a RSD of less than 2%, exact with a recovery percentage of 98.7% and quantification limits of 0.72 and 1.56 fig / mL were established for the system and method respectively. According with this results, the analytical methodology is adequate to evaluate the in vitro permeation of benzoic acid, in polyelectrolyte complexes, through porcine skin in Franz cells.

Keywords : Vitamin C; titrimetry; content determination; quality control.

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