Abstract

AVILA AVILA, Wilson; MEDINA VARGAS, Oscar J.  and  MORENO BASTIDAS, Lucía M.. Application of a 2³ factorial design in the assisted extraction by microwave and evaluation of the antioxidant activity of the compounds present in Vaccinium meridionale fruits. Rev. colomb. cienc. quim. farm. [online]. 2019, vol.48, n.3, pp.643-661.  Epub Nov 03, 2020. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v48n3.84983.

Blueberry (Vaccinium meridionale) forms part of the family Ericaceae; it is a fruit rich in flavonoids and anthocyanins (polyphenols), which have antioxidant properties and also shows some properties suitable for inhibition of free radicals. A 23 factorial design was performed to investigate the variables effects: temperature, time and the ethanol percentage %, on the antioxidants extraction process by Microwave Assisted Extraction (MAE). The extraction conditions were: temp (343.15-383.15 K), time (5-15 min), ethanol (0- 80%). A factorial design was performed, with a total of 8 experiments, allowing to determine the influence of the relation of the variables related to the extraction process of compounds with antioxidant activity by DPPH- method, getting a first order factorial model with interactions and a 100% reliability level and a R2 =1. The experimental design and evaluation of the results were performed with the software R Studio® free version.

Keywords : DPPH-; Vaccinium meridionale; MAE; factorial design; antioxidants.

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