Abstract

GIRALDO, Johnnatan Mauricio et al. Determination and qualitative comparison of cannabinoids present in commercial natural products of the Cauca Department-Colombia. Rev. colomb. cienc. quim. farm. [online]. 2019, vol.48, n.3, pp.789-810.  Epub Nov 04, 2020. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v48n3.84992.

Cannabis sativa has now experienced an increasing interest in the study of its components since it is attributed therapeutic properties in the treatment of diseases. In Colombia and specifically in the Cauca Department, Cannabis products are marketed both for non-medicinal and therapeutic purposes. Consequently, it is necessary to analyze these products in such a way that the composition of the products and their possible effect on health can be known. The analysis ofthe components of these products was carried out using high performance liquid chromatog-raphy (HPLC) and mass spectrometry (MS), in such a way that they allowed the identification of the main cannabinoid species; Δ9-tetra hydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG). The separation of the analytes was carried out by means of the implementation of a reverse phase C18 analytical column, isocratic elution 1 mL/min, system pressure 800 PSI, a mixture of acetonitrile ACN and phosphate buffer (KHPO4) in relation 65/35 as mobile phase, injection volume of10 µL, analysis time of15 min, and detection at 220 nm.

Keywords : High performance liquid chromatography (HPLC); mass spectrometry (MS); delta 9 tetrahydrocannabinol (Δ⁹ THC); cannabinol (CBN); cannabigerol (CBG); cannabidiol (CBD).

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