Abstract

HERNANDEZ-QUELLAR, Camilo Eduardo; CASTRILLON-MARTINEZ, Esteban  and  GALLEGO-GOMEZ, Juan Carlos. Drug discovery based on live cell imaging. Rev. colomb. cienc. quim. farm. [online]. 2020, vol.49, n.1, pp.114-136.  Epub Nov 10, 2020. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v49n1.87026.

The efficient compounds' design taking advantage of the molecule's structural characteristics and efficient search for therapeutic targets has provided effective tools for the research of new treatments when this is focused on disease cellular mechanisms. Phenotypic changes produced by in vitro interaction between molecules and targets can be monitored quantitatively by live cell imaging. To guarantee adequate interaction, it is necessary to consider different crucial elements: 1. Structural characteristics and molecular dynamics of the evaluated compound. 2. Target relevance for the concern physiopathology. However, overview's ignorance of the drug discovery, from structural and cellular problems, has slowed the new treatments research. This literature review presents some important structural aspects for compounds' characterization as therapeutic candidates and experimental approaches for cellular systems development. Subjects discussed are focused on live cell imaging and we also provide relevant examples. Phenotypic monitoring of interactions' produced effects between the chemical candidate and therapeutic target in a cellular system can favor the efficient search of potentially therapeutic molecules.

Keywords : Drug discovery; confocal microscopy; in vitro techniques; molecular structure; biomarkers; molecular probes.

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