Abstract

FONSECA GOLA, Antonio; CORDOVI VELAZQUEZ, Juan Manuel; BENITEZ GUERRA, Niurka  and  FERNANDEZ CERVERA, Mirna. Development and validation of a gas chromatographic method for the determination of menthol, methyl salicylate, thymol, and benzoic acid in antiseptic solution. Rev. colomb. cienc. quim. farm. [online]. 2020, vol.49, n.2, pp.306-328.  Epub Nov 26, 2020. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v49n2.89484.

A method was developed and validated by gas chromatography for the simultaneous quantification of menthol (MT), methyl salicylate (SM), thymol (TM) and benzoic acid (AB) in an oral antiseptic solution. A DB-WAX column and diethylene glycol was used as internal standard. Linearity was determined in a concentration range of 64.00 to 96.00 µg/mL (MT), 80.00 to 120.00 µg/mL (SM), 96.00 to 144.00 µg/mL (TM) and 48.00 to 72.00 µg/mL (AB) achieving correlation coefficients greater than 0.999, and coefficients of variation of the response factors of 1.18, 1.95, 3.52 and 1.48%, respectively. Detection limits were established: 0.51, 1.14, 3.34 and 1.402 ng/mL for MT, SM, TM, and AB, respectively, while the quantification limits were 1.45, 3.43, 9.73 and 4.36 ng/mL in each case. Recovery percentages were 100.03, 99.31, 99.92 and 100.12; with coefficients of variation of 0.42, 0.79, 0.66 and 0.76% for each case. The method was linear, accurate, precise, and selective for the determination of analytes in quality control.

Keywords : Benzoic acid; gas chromatography; menthol; methyl salicylate; thymol; validation of analytical methods.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )