Abstract

GOMES DA SILVA FERREIRA, Rosilene et al. The selective obtaining of amyrins from Amazonian Protium oleoresins. Rev. colomb. cienc. quim. farm. [online]. 2020, vol.49, n.2, pp.482-497.  Epub Nov 23, 2020. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v49n2.89923.

The oleoresin produced by species of genus Protium sp. is rich in alpha and betaamyrins, two triterpenes with many pharmacogical activities. Considering the need to make the improved obtainment of these products feasible, this study sought to optimize techniques for the extraction and isolation of amyrins from resin. Two methods of extraction (maceration and sonication) with different solvents were compared to direct isolation from crude resin. The isolation of triterpenes was performed by chromatographic columns and the yields of extracts and fractions were analyzed by analysis of variance. The best extraction solvent for amyrins was hexane for both maceration and sonication methods (38.16±2.06% and 37.67±8.21%, respectively). There was no statistical difference between these methods and the direct method (32.05±2.40%). Additionally, the direct method is cheaper and more environmentally friendly. Thus, this study showed that it is possible to obtain a large quantity of amyrins by means of cheap, fast and ecological methods.

Keywords : Protium; alpha and beta-amyrins; yielding extraction; extraction optimization.

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