Abstract

SAAVEDRA PLAZAS, Diana Carolina; PETTERLE, Ricardo  and  PIECHNIK, Cláudio Adriano. The model of artificial ovary as a cellular test to evaluate the safety of Spirulina máxima and Kefir extracts: a preliminary study. Rev. colomb. cienc. quim. farm. [online]. 2020, vol.49, n.3, pp.759-775.  Epub Feb 25, 2021. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v49n3.91342.

S. maxima and Kéfir are known and used for their antioxidant and immunostimulant properties. This study aimed to evaluate the natural extracts of these two agents with the technique of manipulation of oocytes included in preantral follicles (MFP), because the technique can replace the use of laboratory animals and could also harmonize the laws that try to reduce and improve the use of animals for the study of new drugs and to integrate good laboratory practices. The natural extracts studied were obtained from S. maxima and Kefir, both substances are known in the market for their antioxidant and immunostimulating activity. Both were evaluated in suspensions of 10, 50, 100 and 200 µg.mL-1. The results show that Spirulina produced a decrease in survival, development, and follicular diameter. While Kefir did not show positive or negative influences on the growth and development of preantral follicles, only the concentration of 200 µg.mL-1 decreased follicular survival. The MFP technique proved to fit into the 3R policy (replacement, reduction, and refinement) and allowed to evaluate cytotoxicity, showing that the technique can be used as a safety test in natural extracts.

Keywords : Non animal testing; adverse effect; dietary supplement; prebiotic; wellness; preantral follicle.

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