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Revista Colombiana de Ciencias Químico - Farmacéuticas

versão impressa ISSN 0034-7418versão On-line ISSN 1909-6356

Resumo

SORO, Ariadna Soledad; VALENZUELA, Gabriela Malena  e  NUNEZ, María Beatriz. Antioxidant activity of four plant species in the Argentine Northeast. Rev. colomb. cienc. quim. farm. [online]. 2021, vol.50, n.1, pp.236-252.  Epub 05-Nov-2021. ISSN 0034-7418.  https://doi.org/10.15446/rcciquifa.v50n1.95457.

Objectives:

To determine the phytochemical composition and evaluate the anti-oxidant activity of the hydroalcoholic extracts of S. haematospermum; P. niruri; P. tenellus; E. serpens.

Methodology:

The phytochemical composition was analyzed by thin layer chromatography and the antioxidant activity was observed qualitatively by autographic assay. Total phenols were quantified with the Folin-Ciocalteu method and total flavonoids with the aluminum complexation reaction. To determine the antioxidant activity, the discoloration techniques of the free radical DPPH• (2,2-diphenyl-1-picrilhydracil) and of the cation radical ABTS•+ (2,2-Azino-bis-3-ethylbenzothiazolin-6-sulfonic acid) and the reducing power of the iron (III) cation.

Results:

The phytochemical study showed the presence of bioactive metabolites in all species. The total phenol content ranged from: 627.71 ± 1.45 to 369.8 ± 52.6 mg EAG / g sample and total flavonoids was 12.2 ± 0.1 to 4.5 ± 0.3 mg Q / g sample. The species S. haematospermum and P. tenellus presented higher content of polyphenols. The antioxidant activity tests allowed recognizing that S. haematospermum and P. tenellus have a capacity for greater inhibition of the tested free radicals and the hydroalcoholic extracts S. haematospermum and P. niruri showed greater reducing power.

Palavras-chave : Free radicals; polyphenols; Euphorbiaceae; Phyllanthaceae.

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