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Revista Colombiana de Ciencias Químico - Farmacéuticas
versão impressa ISSN 0034-7418versão On-line ISSN 1909-6356
Resumo
MENDEZ TIBAMBRE, María Elizabeth et al. LAMP method as a diagnostic alternative for the detection of the SARS-CoV-2 virus. Rev. colomb. cienc. quim. farm. [online]. 2021, vol.50, n.3, pp.633-649. Epub 22-Mar-2023. ISSN 0034-7418. https://doi.org/10.15446/rcciquifa.v50n3.10020.
Introduction:
The SARS-CoV-2 coronavirus, that causes the COVID-19 disease, has become a global public health problem that requires the implementation of rapid and sensitive diagnostic tests.
Aim:
To evaluate and compare the sensitivity of LAMP assay to a standard method and use RT-LAMP for the diagnosis of SARS-CoV-2 in clinical samples from Colombian patients.
Methods:
A descriptive and cross-sectional study was conducted. A total of 25 nasopharyngeal swab samples including negative and positive samples for SARS-CoV-2 were analyzed, through the RT-LAMP method compared to the RT-qPCR assay.
Results:
LAMP method detected ~18 copies of the N gene, in 30 min, evidenced a detection limit similar to the standard method, in a shorter time and a concordance in RT-LAMP of 100% with the results.
Conclusions:
RT-LAMP is a sensitive, specific, and rapid method that can be used as a diagnostic aid of COVID-19 disease.
Palavras-chave : Molecular diagnostic; RT-LAMP; COVID-19; SARS-CoV-2.
