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Revista Colombiana de Ciencias Químico - Farmacéuticas
versión impresa ISSN 0034-7418versión On-line ISSN 1909-6356
Resumen
MARQUES MACHADO, Tallita et al. Bioassay-guided isolation of α-glucosidase inhibitors from Byrsonima garcibarrigae Cuatrec. Rev. colomb. cienc. quim. farm. [online]. 2022, vol.51, n.3, pp.1114-1130. Epub 30-Dic-2023. ISSN 0034-7418. https://doi.org/10.15446/rcciquifa.v51n3.96982.
Introduction:
Byrsonima garcibarrigae is an endemic tree of Amazonas state, Brazil, with pharmacological and chemical knowledge poorly understood.
Aim:
To investigate the antidiabetic potential of the B. garcibarrigae stem bark.
Methods:
The stem bark was sequentially extracted by maceration with hexane (EHBG), ethyl acetate (EABG), and methanol (EMBG). The antioxidant capacity, α-glucosidase inhibitory potentials and anti-glycation capacities were evaluated. A bio-guided fractionation gave compounds that were characterized by MS and NMR.
Results:
8 compounds were identified by HPLC-MS. EMBG showed the highest α-glucosidase inhibitory activity (1.09±0.32 μg/mL), antioxidant activity (9.2±0.23 μg/mL) and phenolic compounds content (61.43±0.50%), thus was fractionated producing hexane (FHX), chloroform (FCL) and hydromethanolic (FHM) fractions. After additional anti- α-glucosidase assays, FHM (1.02±0.49 μg/mL) was fractionated giving quercitrin and epicatechin. The anti-glycation assay showed that EMBG, FHM and quercitrin presented higher activities in comparison to the positive control, amino-guanidine.
Conclusions:
B. garcibarrigae displayed antidiabetic potential since inhibited α-glucosidase, as well as presented expressive antioxidant and anti-glycation activities were recorded.
Palabras clave : antidiabetic; oxidative stress; Byrsonima garcibarrigae.