Introduction

Dental caries is associated to dysbiosis of dental biofilm. One of the most effective ways to prevent or control this disease is the use with mouthwashes. However, there is a great diversity of these products on the market, and there are few studies that determine their antimicrobial activity in biofilms.

Aim

To evaluate antimicrobial activity of 4 commercial mouthrinses against 4 microorganisms in vitro biofilm model on HAp discs.

Methods

The microorganisms included were S. mutans ATCC 35668, S. salivarius ATCC 13419, S. sanguinis ATCC 10556 and C. albicans ATCC 14053. Biofilms (in individual format and in binomial consortium) were developed on HAp discs for a period of 24 hours and they were subjected to two treatments with the four rinses, one with immediate effect (TEI- 5 minutes) and another residual (TER- 55 minutes).

Results

The antimicrobial activity of all the mouthrinses was very similar. In mono and polymicrobial biofilms, TEI and TER were located, respectively, in inhibition ranges from 91.9 to 100%, and from 97 to 100%.

Conclusion

All mouthwashes showed excellent antimicrobial activity on mounted biofilms, with inhibition values greater than 91.9% in the TEI treatment and 97% in the TER treatment. The best rinses were the identified as one and two, and the greatest action of these was on C. albicans.