Abstract

GARCIA, Dabeiba A et al. Genotyping the human papilloma virus (HPV) in Cytobrush samples taken from patients attending different hospitals in Bogotá (Colombia) and evaluating agreement between two PCR-based methods. Rev Colomb Obstet Ginecol [online]. 2010, vol.61, n.4, pp.310-318. ISSN 0034-7434.

Objective: genotyping HPV in samples taken from patients having different histopathological degrees of cervical injury. Materials and methods: this was a cross-sectional study of cervical scrape samples taken from females having different degrees of histopathological cervical injury in Bogotá during 2009. Luminex^® xMAP^® was used, which is a highly sensitive, reproducible and automated technique, as well as reverse line blot (RLB). Results: infections caused by HPV types 16 and 18 were the most frequently found types of pathology of the cervix. Mixed infections predominated (78.04%); amongst cases having negative pathology, 85.7% was found for cervical intraepithelial neoplasia (CIN) I, 81.25% for CIN II, 78.78% for CIN III and 71.42% for cervical cancer. Agreement between both methods was 67%. Conclusion: infection by VPH types 16 and 18 predominated in the population being studied.

Keywords : papilloma virus; cervical dysplasia; pathology; molecular biology.

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