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Revista de la Facultad de Medicina

Print version ISSN 0120-0011

Abstract

MOCKUS, Ismena; CAMINOS, Jorge Eduardo; ARANZALEZ, Luz Helena  and  RAMIREZ, Doris. Overweight and adiponectin receptors expression in peripheral blood monocytes. rev.fac.med. [online]. 2007, vol.55, n.4, pp.215-223. ISSN 0120-0011.

Background. Previous research had shown that adipose tissue increase is associated to greater atherosclerosis and have also demonstrated adiponectin receptors (AdipoR1 and AdipoR2) expression in atherosclerotic plaque. In addition, previous research have allowed to conclude that atheroma´s macrophage arise from circulating monocytes. Aims. To determine AdipoR1 and AdipoR2 relative expression in peripheral blood monocytes from overweight and normal subjects. Materials and methods. AdipoR1 and AdipoR2 relative expression was determined in peripheral blood monocytes by using real-time polymerase chain reaction. Peripheral blood monocytes were isolated by means of immunoaffinity technique from a group of students aged 18 to 25 years (n=48). Anthropometric and biochemical parameters (total and HDL-cholesterol, triglycerides and insulin resistance estimated by the homeostasis model assessment ratio) were measured. Results. AdipoR1 expression in peripheral blood monocytes was higher than that of AdipoR2 (p < 0.001). According to sex, body mass index, percentage body fat, insulin resistance or lipid profile, there were no differences in AdipoR1 or AdipoR2 expression. Conclusions. Our results about a greater AdipoR1 expression in comparison to AdipoR2 corroborate previous findings. Our data suggest no relation between the expression of AdipoR1 and AdipoR2 in monocytes of normal and overweighted subjects and insulin sensibility. Further studies are recommended which involve population with body mass index superior to 30 kg/m2 and individuals with abdominal obesity, and in addition establish possible effects of style-life related factors on the regulation of the expression of these receptors in peripheral monocytes.

Keywords : adiponectin; overweight; monocytes; reverse transcriptase polymerase chain reaction; gene expression; receptors.

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