Resumo

PULIDO MORERA, Diana Rocio; ACOSTA LOSADA, Orlando  e  GUERRERO FONSECA, Carlos Arturo. Increase of heat shock cognate protein, HSC70, in MA104 cells following rotavirus infection. rev.fac.med. [online]. 2007, vol.55, n.4, pp.224-239. ISSN 0120-0011.

Background. HSC70 acts as a cellular coreceptor for rotavirus particles during the infection of MA104 cells, but changes in the accumulation levels of this protein through the rotavirus infection cycle are unknown. Objetive. To determine if the accumulation levels of HSC70 in MA104 cells change during the infection by rotavirus. Materials and methods. Immunofluorescene microscopy, Western blotting an ELISA were used in order to determine HSC70 accumulation levels in MA104 cells after infection (0 up to 16 h p.i.) by rotavirus strains Wa (human), Rf (bovine) and RRV (simian). Additionally, a rabbit polyclonal serum against purified rotavirus particles was used for detecting rotavirus antigen in an immunoperoxidase assay. Results. The immunofluorescence staining of cells infected with each of three rotavirus strains showed a direct correlation between the increase of fluorescence in the cytoplasm and the increase of the viral antigen. The intensity of the cytoplasm fluorescence was 1.1 to 2.0 times higher in infected cells than that observed in control cells. On the other hand, the intensity of the fluorescence in the cell membrane was similar to that of the non-infected control cells. The Western blotting assay did not allow to establish measurable differences between infected and non-infected MA104 cells, because the increase of the protein are below the detection limit. According to the results of ELISA, HSC70 level increases 1 to 3 times in MA104 cells after (8 h p.i.) the infection with strain RRV, whereas infection with strain RF produced an increase which ranged from 1.3 to 2.5 times after 10 h p.i. Infection by strain Wa was correlated with un increase of HSC70 level between 1.7 and 3.0 times after 2 h p.i. Conclusions. The results suggest that the increase of HSC70 accumulation level possibly corresponds to an increased expression of its gene during the rotavirus infection and probably it is involved in rotavirus multiplication steps additional to those of entry and exit processes.

Palavras-chave : HSC70 Heat-shock cognate protein; rotavirus infection; flourescent antiobody technique direct; antibody formation.

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