Resumo

VASQUEZ P, CARMEN A.; SALDARRIAGA . O, YAMILLE  e  CHAVERRA R, DUVERNEY. Susceptability of fifth instar nymphs Rhodnius prolixus (Hemiptera Reduviidae) to the action of the fungus Beauveria bassiana . Rev. Colomb. Entomol. [online]. 2005, vol.31, n.1, pp.15-19. ISSN 0120-0488.

The effect of the entomopathogenic fungus Beauveria bassiana (strain UdeA₁₃) was evaluated on fifth instar nymphs of Rhodnius prolixus under laboratory conditions. The strain was isolated from fi. pallesceris in the region of San Onofre (Sucre) and cultivated on Sabouraud Dextrosa Agar (SDA). The insect colonies were propagated and reared under controlled conditions of ternperature (25 27°C) and relative humidity (80%) in darkness for growth and reproduction. The insects were feed weekly for 1 h with hen’s blood (Gallus gaitas). The treatments consisted of conidial suspensions of the entomopathogenic fungus B. bassiana at concentrations of 3x10⁵, 1x10⁷, 3x10⁸ y 3x10⁹ conidia/ml which were sprayed on the insects using the Pulverization Tower emulator system Control insects were sprayed with an aqueous solution of Tween 80 (0,0l%). Effects were compared among fungus treatments and the controls Significative differences (P0,01) were found among ah treatments regardless of whether the control group was included in the analysis The concentrations of 3x10⁶ y 3x10⁹ conidia/ml produced 90 to 100% of mortality between 7 and 9 days after treatment. The results of this research indicate that fi bassiana (UdeA13) can be considered as a possible agent for the biological control of the vector R. prolixus.

Palavras-chave : Biological control; Conidiation Entomopathogenic; fungus.

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