Abstract

CHAVEZ G, JULIO CÉSAR; ROLDAN R, JUDITH  and  VARGAS V, FRANKLIN. Levéis of resistance to two insecticides in populatíons of Aedes aegypti (Díptera: Culicidae) from Perú. Rev. Colomb. Entomol. [online]. 2005, vol.31, n.1, pp.75-78. ISSN 0120-0488.

The purpose of this study was to determine resistance levéis to temephos and deltametrine in two natural populations of Aedes aegypti from Perú. Bioassays in larvae and adults were carried out following the methodology of the World Health Organization. The visualization of Besterase bands was made by poliacrylamide gel electrophoresis (PAQE) in fourth instar larvae. Natural populations from Sullana (Piura) and El Porvenir (La Libertad) presented resistance ratios to temephos of 1.67 X and 1.45 X, respectively, indicating susceptibility in both populations; for deltametrine the Sullana population presented resistance with a KDT₅₀ of 106 min and 68% mortality at 24 h; in the El Porvenir population susceptibility was observed, with a KDT_S0 of 36.9 min and 99% mortality. Esterase B., was identified with a Rf of 0.23 only in El Porvenir population. The insecticide temephos can continué being used in control programs of the Aedes aegypti vector because the species is still susceptible to this organophosphorate; on the contrary with deltametrine, where its effects should be evaluated in natural populations since some of them should be presenting resistance, as in the case of the Sullana population.

Keywords : Bioassays; KDT₅₀; Besterase.

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