Abstract

ACERO, Víctor et al. Interaction of Leishmania (L) chagasi with the Lulo cell line in different environmental conditions. Rev. Colomb. Entomol. [online]. 2006, vol.32, n.2, pp.165-171. ISSN 0120-0488.

There are unknown or poorly understood events in the Leishmania-host cell interactions that merit studies in new in vitro models. The aim of the present work was to evaluate Leishmania chagasi interaction with a cell line (Lulo) previously established and derived from Lutzomyia longipalpisi embryonic tissues, bearing different temperatures and CO₂ concentrations in mind and morphometric changes of the cells. The J774 cell line was used as positive control of the infection. The L. chagasi MH/CO/84/CL-044B strain was used for infection assays. The parasites were added to adherent cells in 5:1 ratio, incubated at 28°C and 37°C with the presence or absence of CO₂. A morphometric study of cells was carried out, before and after infection. Both parasite and cell ultrastructure characteristics were analyzed by electron microscope. The greatest percentage of infection in the Lulo cells at 28°C was registered at 28°C (26,8%) on day six, while at 37°C, in CO₂ absence, the maximum percentage (30,4%) was obtained the ninth day. Morphometric analysis showed a significant increase in the size of Lulo cells infected at 28°C and J774 cells at 37°C with the presence and absence of CO₂ respectively.

Keywords : Ultrastructure characteristics; Temperature and CO2; Promastigote; Percentage of infection; Cell morphometry.

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