Abstract

ROSERO, DORIS A et al. Optimization of a DNA extraction procedure for anopheline mosquitoes. Rev. Colomb. Entomol. [online]. 2010, vol.36, n.2, pp. 260-263. ISSN 0120-0488.

A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

Keywords : Anopheles; PCR; Potassium Acetate.