Abstract

BELTRAN-BEACHE, Mariana; DELGADO-ORTIZ, Juan Carlos; OCHOA-FUENTES, Yisa María  and  CHAVEZ, Ernesto Cerna. Genetic variation of Bactericera cockerelli Šulc. (Hemiptera: Triozidae) suggests new haplotype in México. Rev. Colomb. Entomol. [online]. 2022, vol.48, n.2, e11094.  Epub Sep 06, 2022. ISSN 0120-0488.  https://doi.org/10.25100/socolen.v48i2.11094.

A new genetic variation and potential new haplotype of Bactericera cockerelli was identified based on the analysis of the mitochondrial region Cytochrome Oxidase subunit I of two populations from greenhouses in Villa Unión, Durango and Saltillo, Coahuila, Mexico. A variation was found in the base 247 of a 500 bp amplified of the mtCOI gene, this change implies the substitution of an adenine (A) to a Guanine (G), this mutation was detected in the insects collect from greenhouses and in their progeny obtained under laboratory conditions. This genetic variant has a great potential to be a new haplotype of B. cockerelli, therefore, it was designated Mexico Haplotype 1 (MXH1) with GenBank accession number KX130767. A total of ten insects from the original collection and then from laboratory cages were evaluated for Lso infection and all tested negative.

Keywords : Lso; mtCOI; MXH1; polymorphism; potato psyllid.

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