Abstract

SALGADO, Roger D; RUGELES, Clara C  and  ALVAREZ, Jaime. Heparin and spermatic concentration effect over the percentage of in vitro fertilization of bovine oocites. Rev Colom Cienc Pecua [online]. 2005, vol.18, n.2, pp.122-126. ISSN 0120-0690.

The objective of this study was to evaluate the effect of different heparin concentrations and of the semen in the percentage of bovine oocytes in fertilization medium. In the experiment 1, the obtained oocytes of slaughterhouse ovaries were matured in vitro and distributed at random in six treatments for fertilization in vitro, according to the heparin concentration (2, 4, 8, 10, 16, 20 µg/ ml). In the experiment 2, once selected the heparin concentration from experiment 1, three sperms doses were evaluated in fertilization medium (0.5x10⁶ espermatozoas/ml, 1x0⁶ espermatozoas/ml and 2x0⁶ espermatozoas/ml). In each experiment totally randomized design was used and data were analyzed by ANAVA. The concentration of heparin (p<0.05) affected the percentage of fertilized oocytes being the best results in the concentration of 10 µg/ml, with a rate of penetration of 67%. The spermatic concentration affects the fertilization, the biggest oocytes proportion with normal fertilization (74%) when using concentration 2x10⁶ spermatozoas/ml. However, it was not significantly different to the concentration of 1x 10⁶ (p>0.05). The results indicate that the fertilization rate keeps direct relationship with the heparine dose and the spermatic concentration.

Keywords : bovine; fertilization in vitro; heparin; swim up.

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