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Revista Colombiana de Ciencias Pecuarias

Print version ISSN 0120-0690
On-line version ISSN 2256-2958


CRUZ-CASALLAS, Pablo E; VELASCO-SANTAMARIA, Yohana M  and  MEDINA-ROBLES, Víctor M. Determination of spermatocrit and effect of volume of insemination dose on fertility in yamú (Brycon amazonicus). Rev Colom Cienc Pecua [online]. 2006, vol.19, n.2, pp.140-145. ISSN 0120-0690.

Yamú (Brycon amazonicus - Spix & Agassiz 1829) is an omnivorous, fast- growing fish with excellent meat quality, given it commercial importance. To standardize the spermatocrit (SPCT), 23 samples of semen were centrifuged at 14000 g during 1, 3, 5, 7 or 10 min. The centrifugation time had a significant effect on SPCT value (p<0.05). With the aim to know the sperm concentration (SC) throughout the reproductive season and its relationship with the SPCT, 40 males were treated with carp pituitary extract (4.0 The SC was evaluated by Neubauer chamber (dilution 1:1200) and SPCT by centrifugation at 14000 g during 5 min. The relationship between SPCT and SC was positive and highly significant (y= 1.69 + 0.59x; r2= 0.74; p <0.0001), but statistical differences were not observed (p>0.05) among the months evaluated (march, april and may). Finally, to determine the effect of volume of seminant dose on fertility, 2 g of eggs were seminated with 3, 15,45, 90 or 180 µL of semen (n= 12). The SC was previously adjusted to contain 50000 sptz/egg, using as extender seminal plasma homologous. The fertilization rate were 83, 86, 86, 85 and 87%, respectively (p>0.05).

Keywords : seminant dose; spermatocrit; sperm concentration; yamú.

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