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vol.19 issue2Determination of spermatocrit and effect of volume of insemination dose on fertility in yamú (Brycon amazonicus)Evaluation of different cryoprotectors for cryopreservation of yamú (Brycon amazonicus) spermatozoa author indexsubject indexarticles search
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Revista Colombiana de Ciencias Pecuarias

Print version ISSN 0120-0690
On-line version ISSN 2256-2958


CRUZ-CASALLAS, Pablo E; MEDINA-ROBLES, Víctor M  and  VELASCO-SANTAMARIA, Yohana M. Protocol for cryopreservation of yamú: Brycon amazonicus. Rev Colom Cienc Pecua [online]. 2006, vol.19, n.2, pp.146-151. ISSN 0120-0690.

semen Cryopreservation is an important tool for gametes storage during indefinite time, especially in species whose habitat and population are endangered. This is an alternative to optimize the breeding process in captive fish, especially in species with a gonadal maturation asynchronous and seasonal reproductive cycle like yamú (Brycon amazonicus - Spix & Agassiz 1829), a characidae fish distributed in Amazon, Orinoco and Essequibo river basins. This report presents the protocol for semen cryopreservation in this species, it was obtained after the evaluation of different cryoprotectants, freezing curves and packaging volume. The efficient of this protocol has been demonstrated in several laboratory and fish farm experiments, with similar fertility rates to obtained with fresh sperm, being an important methodological tool for seminal cryopreservation in yamú.

Keywords : cryopreservation; fertility; semen; tropical fish; yamú.

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