Abstract

VELASCO-SANTAMARIA, Yohana M; CORREDOR-SANTAMARIA, Wilson  and  CRUZ-CASALLAS, Pablo E. Effects of the conservation system on eggs fertility of yamú (Brycon amazonicus) during shortterm storage.. Rev Colom Cienc Pecua [online]. 2006, vol.19, n.2, pp.167-174. ISSN 0120-0690.

The aim of this study was to evaluate the effects of the conservation system on eggs fertility of yamú (Brycon amazonicus) during short-term storage. Mature yamú females and males were induced to stimulated ovulation and spermiation by injection of carp pituitary extract. The eggs obtained from the same female were subjected to three different conservation systems: in situ conservation (IS, in the ovarian cavity), ex situ at room temperature (T°E) and ex situ at refrigeration temperature (T°R). The egg diameter (ED) and egg viability were evaluated at different times of storage (0 - control-, 15, 30, 60 and 120 min). The ED was measured with a rule coupled to the stereomicroscope objective, and the viability was evaluated by fertility test; for this purpose, three g of eggs were inseminated with 250 mL of fresh semen. The total larvae length and the volume of the egg yolk were measured, too. In the three conservation system, the ED increased through the time, being higher than the control (p<0.05) from the 15 min of storage. The highest ED values were observed in eggs storage at TºR (p<0.05). Up to 60 min of storage, in IS and T°E conservation system, the fertilization rate with respect to control did not vary (p>0.05). In all conservation system at 120 min of storage, the fertilization rate was smaller than the control; however, in the TºR conditions the fertilization percentage decreased drastically after 30 min, being smaller (p<0.05) than the control. Although the larvae did not show morphological alterations, the larvae control were longer than the other conservation system (p<0.01). In conclusion, the ovulated eggs of yamú could maintain its viability up to 60 min postovulation in the ovarian cavity or at room temperature. In contrast, the refrigeration produced a drastic reduction of egg fertility in this species.

Keywords : eggs; fertility; storage; temperature; yamú.

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