Abstract

MONDRAGON, Néstor; VERA, Víctor  and  RESTREPO, Guillermo. Evaluation of foot and mouth disease oil bivalent vaccines (O1 Campos and A24 Cruzeiro) produced by different purification and concentration methods.. Rev Colom Cienc Pecua [online]. 2006, vol.19, n.4, pp.373-381. ISSN 0120-0690.

This project evaluated two methods for viral concentration and purification and the effect of antigenic mass of Foot and Mouth Disease Virus (FMDV) in the capacity of bivalent vaccine products (A24 Cruzeiro y O1 Campos) to induce antibodies against proteins associated to the capsid (CP) and proteins non associated to the capsid (NCP) of the virus, in cattle. Groups of 8 bovines (aged 12 to 24 months ) were immunized on day 0 an 30 with one of four vaccines that were formulated with a different viral load (vaccine 1, 16.9; vaccine 2, 8.8; vaccine 3, 17.9 and vaccine 4, 7.7 ug/dose). The antibody response against CP detected by ELISA CF,L was evaluated on days 30 and 60; the reactivity against NCP was measured by ELISA-I 3ABC/EITB. Antigens treated with salts induced higher reactivity against CP compared those treated with PEG. One of the animals vaccinated with salt treated antigens was positive to NCP. We found that different results could be obtained depending of the process for antigen concentration and purification:  virus treated with salts, with either a high or a low  antigenic load (vaccines 3 and 4) induced a strong immunity, but with a high antigenic load  there is a major risk of inducing reactivity against NCP. In the PEG treatment (vaccines 1 and 2), we obtained good protection, without interference in the assessment of the infected animals when they were evaluated by ELISA-3ABC-I/EITB for epidemiological purposes.

Keywords : antigenic mass; Food and Mouth Disease Virus (FMDV); perceptual expected protection (EPP); proteins associated to the capsid (PC); proteins non associated to the capsid (PNC).

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