Revista Colombiana de Ciencias Pecuarias
Print version ISSN 0120-0690
BARRERA, Daniel I; GIRALDO, Jorge H; CARLOS M, Duque and ARBELAEZ, Luis F. Electrophoretic identification of α2-macroglobulin in the plasma of tropical hair sheep (Ovis aries) and buffalo (Bubalus bubalis). Rev Colom Cienc Pecua [online]. 2007, vol.20, n.3, pp. 304-311. ISSN 0120-0690.
Blood plasma from six different non pregnant and pregnant species, including human blood plasma, was analyzed for detection of α2-Macroglobulin (α2-M). The tropical hair sheep (Ovis aries) and the buffalo (Bubalus bubalis) were studied for the first time in Colombia. The presence of the α2-M in plasma of all the species was demonstrated by SDS 7.5% PAGE as bands of 180 kDa as well as by non-denaturing 5% PAGE with bands of 720 kDa. The tetrameric form α2-M (tetramérica) and the pregnancy zone protein (PZP) (dimeric) purified at 98%, as well as its corresponding bans from human plasma were used as control. The N-terminal sequence of the band of 180 kDa in Tropical hair sheep plasma was very similar to the purified human α2-M. The results indicated the presence of α2-M in blood plasma of all the species tested, while the PZP was present only in the pregnant human plasma. Both human and bovine α2-M became activated with the fast form by reacting with Methylamine. This Fac. demonstrates the differences in the reactivity of the animal’s α2-M with primary amine as compared with the human α2-M. It could be necessary to unify purification methods into one method for all species, so that the sensitive domain of the α-macroglobulins (thiolester and bait region) receives the same treatment and grade of denaturation for all α2-M preparation.
Keywords : α2-macroglobulin; Bubalus bubalis; Ovis aries; SDS PAGE.