Abstract

LENIS, Gustavo A et al. Induced breeding and production of fingerlings of Sabaleta Brycon henni: Determining the time of latency using Carp Pituitary Extract. Rev Colom Cienc Pecua [online]. 2009, vol.22, n.2, pp.143-155. ISSN 0120-0690.

This research was conducted in the San José del Nus fish farm, located in the Municipality of San Roque (department of Antioquia). This study began in 2002 with the capture and suitability of 100 wild breeding fish batches coming from three sub-basins near to San José del Nus fish farm. In 2003, another 80 specimens were captured and utilized for first hormonal induction trials by stimulating final maturation and ovulation with two doses of Carp pituitary extract (CPE) (0.5 + 5.0 mg/kg B.W.). Dry extrusion was used in the three groups of fish to obtain gametes. Spermiation and final maturation plus ovulation were induced in twenty males and females, respectively. The most relevant result was ovulation and spawning in 8 cases. Two of them were completely successful; their latency period was 13 and 11 h, respectively. From the first previously fertilized female (136.2 g. B.W.) 1421 eggs were extracted, with a survival of 28% from embryo to larva; and 35.7% from larva to alevin. The second female (90 g B.W.) spawned 1348 eggs, resulting in a fertilization rate of 74%, larval survival of 40% and survival until alevin for commercial use of 25%. In both cases, spawning resulted in an alevin net number of 141 and 101, respectively. Water incubation temperature was 24.5 ºC and 25.3 ºC. It is important to emphasize that in the same year a previously spawned female was found, and three months later this female presented gonadal maturity once again.

Keywords : fertilization; hatching; hormonal induction; latency; spawning; viability.