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Revista Colombiana de Ciencias Pecuarias

Print version ISSN 0120-0690
On-line version ISSN 2256-2958

Abstract

CANAS BERMUDEZ, Omaira; QUIJANO PARRA, Alfonso  and  FARBELAEZ RAMIREZ, Luis. Comparative activation and kinetic of plasmin bufaline with the human one. Rev Colom Cienc Pecua [online]. 2010, vol.23, n.1, pp.47-54. ISSN 0120-0690.

The Plasminogen is the zymogene of the Plasmin, enzyme which physiologically is activated by two different enzymes, the tissue plasminogen activator and the urokinase, the plasmin is the enzyme that dissolves blood clots. In this study the human plasmin was compared to the bufaline plasmin, in the activation from the zymogene to the enzyme form as well as in the affinity to the chromogenic substrate. The two plasminogens were purified by the same chromatographies methods: affinity and ion-exchange. Furthermore, both plasminogens were activated by human urokinase. The bufaline plasmin showed more activation and affinity (1.35 mM) that the human plasmin (2.16 mM), in addition, the bufaline plasmin demonstrated a 1.5 times more affinity to the chromogenic substrate that the human plasmin. This study demonstrated that the plasminogens of several species can be purified by this method. Besides, one more time the animal’s plasmins probably to be more efficient in the dissolution of blood clots or degradation of substrates than the human plasmin. More over this study indicated that the bufaline plasmin can be used in clinical determinations of patients with cardiovascular diseases. This also reduces the determination time of fibrinolytic parameters that physicians can give, having more time to take appropriate treatment.

Keywords : fibrinolysis; glu-plasminogen; kinetics; plasmin; urokinase.

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