Abstract

PARK, Inkyung  and  CHO, Jaiesoon. Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1)as a potential feed additive. Rev Colom Cienc Pecua [online]. 2011, vol.24, n.1, pp.11-18. ISSN 0120-0690.

Extracellular proteolytic activity was found in JSP1, an Antarctic bacterial isolate. The strain was related to Bacillus sp, based on 16S rRNA gene sequence analysis. The JSP1 protease was partially purified by ammonium sulfate precipitation. Optimal enzyme activity occurred at 40 C and pH 7.4. Enzyme activity was significantly enhanced in the presence of Mg²⁺ and Ca²⁺ and was completely inactivated in presence of Cu²⁺, Zn²⁺, Hg²⁺, EDTA and SDS. The enzyme hydrolyzed casein the most effectively among the protein substrates tested. The enzyme also exhibited relatively high activity on keratin and gluten, and was active against peptidyl conjugates such asL-Leu-p-Nitroanilide and N-Succinyl-L-Phe-p-Nitroanilide. This study suggests that JSP1 protease could be utilized as a potential environmentally-friendly feed additive in animal production.

Keywords : antarctic, Bacillus sp; feed additive; keratin; livestock production; protease.

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