SciELO - Scientific Electronic Library Online

 
vol.25 issue2Allelic frequency of the Kappa-Casein gene in Colombian and creole cattle breedsGenetic variability of the bovine prolactin-Rsal loci in Holstein cattle in Antioquia province (Colombia) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Revista Colombiana de Ciencias Pecuarias

Print version ISSN 0120-0690
On-line version ISSN 2256-2958

Abstract

C SEPULVEDA, Jeannie  and  ANGEL-MARIN, Paula A. Genetic variability of Senepol cattle in Colombia using molecular markers. Rev Colom Cienc Pecua [online]. 2012, vol.25, n.2, pp.183-190. ISSN 0120-0690.

The Senepol beef cattle breed was introduced into Colombia through the use of artificial insemination and embryo transfer from a small nucleus of animals. Objective: to estimate the genetic variability of Senepol cattle in Colombia by heterologous microsatellites and to estimate gene and genotypic frequencies of single nucleotide polymorphic markers through calpastatin (CAST1), calpain (CALP316), and leptin (PB) genes. Methods: 412 blood samples from 28 herds were genotyped for population genetic structure with the STR: INRA32, BM2113, ETH10, BM1824, INRA037, ETH225, INRA064, SPS115, TGLA126, and TGLA122 microsatellite markers. Three SNPs of calpastatin, calpain, and leptin genes were used. Results: all microsatellites and SNP markers were polymorphic. The number of alleles ranged from 4 (BM1824) to 11 (INRA37), and the observed heterozygosity varied between 0.21 (INRA64) and 0.89 (BM2113). Combined probability of exclusion for the microsatellites was higher than 99.99%, indicating the usefulness of this set of markers for parentage testing in Senepol. Conclusions: despite being a small and closed population, this nucleus presents high genetic variability and low inbreeding.

Keywords : beef cattle; genetic diversity; genetic structure; microsatellite marker.

        · abstract in Spanish | Portuguese     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License