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Revista Colombiana de Ciencias Pecuarias

Print version ISSN 0120-0690
On-line version ISSN 2256-2958

Abstract

C RINCON, Juan; LOPEZ, Albeiro  and  ECHEVERRI, Julián. Genetic variability of the bovine prolactin-Rsal loci in Holstein cattle in Antioquia province (Colombia). Rev Colom Cienc Pecua [online]. 2012, vol.25, n.2, pp.191-201. ISSN 0120-0690.

The Holstein breed, widely used in Antioquia's dairy industry, has undergone genetic changes due to selection that have affected the frequencies of some polimorphisms within important genes. Objective: to identify polymorphism changes in the prolactin RsaI (PRL-Rsal) locus and to characterize the structure and allelic distribution within the Holstein population in Antioquia. Methods: a total of 1.462 Holstein cows from 11 subpopulations (municipalities) of Antioquia were used. The ADN was extracted from leucocytes using the salting-out method and genotyping was performed by PCR-RFLP. Genetic diversity was determined by heterozygosity. The Hardy-Weinberg equilibrium (HW) and the genetic differentiation among populations were performed using the Arlequin 2.0 software. Allelic and genotypic frequencies were assessed with the SAS 9.2 statistical software. Results: the genotypic frequencies found were 0.695 (AA), 0.276 (AB), and 0.029 (BB), while the frequencies of the A and B alleles were 0.833 and 0.167, respectively. There were no deviations from HW equilibrium in any population. Genetic diversity among populations, expressed in terms of heterozygosity, showed a medium value (Ho=0.276). The FST value of the entire population was significant, indicating genetic differentiation. In addition, some matched FST showed high differentiation. The FIT and FIS parameters were not significant, suggesting that population endogamy or exogamy is not occurring. Conclusions: the bovine PRL-RsaI polymorphism is a suitable marker to evaluate characteristics of economic importance and apparently it has not been influenced by selection pressure. In fact, a significantly high variability (p<0.05) in allele frequencies was observed within the Antioquian Holstein cattle subpopulations.

Keywords : genetic differentiation; Hardy-Weinberg equilibrium; PCR-RFLP; single nucleotide polymorphism.

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