Abstract

RODRIGUEZ-MONTES DE OCA, Gustavo A et al. Production of ''Chame'' (Dormitator latifrons, Pisces: Eleotridae) larvae using GnRHa and LHRHa. Rev Colom Cienc Pecua [online]. 2012, vol.25, n.3, pp.422-429. ISSN 0120-0690.

The Pacific fat sleeper is a potential species for aquaculture in Latin American countries. Nevertheless, production depends on wildcaught juveniles, thus needing hatchery produced larvae. Objective: the purpose of this study was to determine the ideal conditions for viable gamete release and larvae laboratory production. Methods: a total of 16 mature male and 16 female fish were allocated to one of four groups (n=4) that were injected with either saline solution, Desgly10-Ala6 LHRHa, salmon GnRHa + domperidone, or implanted with salmon GnRHa. Results: spermiation was observed in all treatments. Spawning rates were 100% at 24 and 48 h for the GnRHa implanted group, 25% for the LHRHa group, and 0% for the salmon GnRHa + domperidone group (48-72 h post injection). Conclusion: GnRHa and LHRHa are a succesful tool for chame induced reproduction. A gross morphological description of oocytes, sperm quality, and first stages of larval development is included.

Keywords : chame fish; gamete release; pacific fat sleeper; spawning; spermiation.

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