Abstract

RAMIREZ-GARCIA, René  and  MALDONADO-ESTRADA, Juan G. Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report. Rev Colom Cienc Pecua [online]. 2013, vol.26, n.3, pp.219-225. ISSN 0120-0690.

Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne's Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months with no response to antibiotic treatment. Consequently, the cow was subjected to euthanasia. Treatment approach: antemortem milk and peripheral blood samples and postmortem colon, mediastinic, mesenteric lymph nodes, and spleen samples were processed for macrophages isolation. Total DNA was extracted from macrophages and used to diagnose IS900 of Mycobacterium avium paratuberculosis (MAP) through real time PCR. The MAP IS900 segment was successfully amplified from cells of all samples, indicating that these cells were MAP-infected macrophages. Conclusion: macrophages of cows suffering from JD can be used for amplification of the MAP IS900 segment.

Keywords : intracellular bacteria; real time PCR; resident macrophages; susceptible host.