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Revista Colombiana de Ciencias Pecuarias

Print version ISSN 0120-0690

Abstract

CORREA-VALENCIA, Nathalia M; RAMIREZ, Nicolás F; BULTE, Michael  and  FERNANDEZ-SILVA, Jorge A. Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd. Rev Colom Cienc Pecua [online]. 2017, vol.30, n.2, pp.101-115. ISSN 0120-0690.  http://dx.doi.org/10.17533/udea.rccp.v30n2a02.

Background:

paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and indirect diagnostic techniques has been of special interest.

Objective:

to report the diagnosis and detection of MAP using several diagnostic tests in a herd of the Northern region of Antioquia, Colombia.

Methods:

serum samples from the study herd were analyzed, using a commercial ELISA (enzyme-linked immunosorbent assay) kit. Fecal samples were cultured by duplicate using Herrold´s egg yolk medium (HEYM), and analyzed by an end- point IS900-specific nested PCR protocol, and a commercial F57-real-time PCR kit.

Results:

eight out of 27 serum samples in the study herd resulted ELISA-positive. None of fecal samples resulted positive to HEYM culture by duplicate and none were found to be positive by F57-real-time PCR. Seven of the 27 fecal samples were found to be positive by end-point IS900-specific nested PCR. Agreement was found between ELISA and end-point IS900-specific nested PCR in one of the animals.

Conclusion:

the present study gives information about the agreement between direct and indirect MAP-detection techniques, using different matrixes from animals under the same husbandry conditions.

Keywords : culture medium; ELISA; Johne´s disease; MAP; molecular diagnosis.

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