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Revista Colombiana de Ciencias Pecuarias
versão impressa ISSN 0120-0690versão On-line ISSN 2256-2958
Resumo
RIOS-ALANIS, Ana M et al. Molecular characterization of bacterial microbiota associated with infectious bovine keratoconjunctivitis in Michoacán, Mexico. Rev Colom Cienc Pecua [online]. 2021, vol.34, n.1, pp.18-28. Epub 14-Jun-2022. ISSN 0120-0690. https://doi.org/10.17533/udea.rccp.v34n1a02.
Background:
The most common ocular disease affecting cattle worldwide is infectious bovine keratoconjunctivitis (IBK), which has been associated with Moraxella bovis bacterium.
Objective:
To report the molecular characterization of the ocular bacterial microbiota and its relation to IBK in cattle in two dairy regions in Michoacán, Mexico.
Methods:
A total population of 761 bovines were evaluated, of which 17 (2.23%) showed symptoms of IBK. Thirty-eight bacterial isolates from ocular samples of bovines with IBK were characterized by Gram-staining and antimicrobial sensitivity. In addition, isolates were identified by sequence comparisons of the 16S ribosomal gene.
Results:
The genus Moraxella was one of the most abundant bacteria and M. bovoculi was the most predominant species.
Conclusion:
The bacterial isolates identified in eye lesions of cattle and associated to IBK are diverse. To the author´s knowledge, this is the first study on the subject in Mexico; therefore, more research is needed to estimate the incidence of IBK and determine its associated microbiota.
Palavras-chave : bacteria; bovine; corneal ulceration; dairy cattle; eye infection; IBK; keratoconjunctivitis; Moraxella bovis; Moraxella bovoculi; ocular bacteria; ocular disease; pink eye.